Recombinant Human IMMT 293 Cell Lysate

• Cat.No.: IMMT-5216HCL

Specification

• Description: Antigen standard for inner membrane protein, mitochondrial (mitofilin) (IMMT), nuclear gene encoding mitochondrial protein, transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: IMMT inner membrane protein, mitochondrial [ Homo sapiens ]
• Official Symbol: IMMT
• Synonyms: IMMT; inner membrane protein, mitochondrial; inner membrane protein, mitochondrial (mitofilin); mitochondrial inner membrane protein; HMP; MINOS2; mitochondrial inner membrane organizing system 2; mitofilin; P87; P89; motor protein; proliferation-inducing gene 4; cell proliferation-inducing protein 52; cell proliferation-inducing gene 4/52 protein; PIG4; PIG52; P87/89; MGC111146; DKFZp779P1653
• Gene ID: 10989
• mRNA Refseq: NM_001100169
• Protein Refseq: NP_001093639
• MIM: 600378
• UniProt ID: Q16891
• Chromosome Location: 2p11.2
• Function: protein binding

Reviews

09/07/2020

High synthesis efficiency, high yield and good stability.

08/04/2020

Complete specifications, suitable for different scale experiments.

01/02/2019

The production quality is very stable, with good repeatability between and within batches.

Q&As

Which members of the mitochondrial inner membrane transporter complex do IMMT directly interact with?

IMMT has direct interactions with other members of the complex such as TOM70 and TOM20.

Whether IMMT role in nervous system development and disease have?

This protein plays an important role in the mechanism of nervous system development and diseases. It can participate in the signal transduction of mitochondrial adaptive response, calcium ion and cell death.

Which diseases is IMMT associated with?

It is associated with mitochondrial diseases or some metabolic disorders, such as mitochondrial fatty acid oxidation deficiency, muscle diseases, and heart disease.

What role do cells play in the IMMT process?

IMMT is involved in coordinating lipid transport between the mitochondrial ER and is responsible for mitochondrial protein transport and assembly.

Are IMMT copy number variations associated with certain diseases?

There may be some associations between IMMT gene copy number variations and metabolism-related diseases and neuropsychiatric diseases, which needs to be further verified.

How IMMT involved in mitochondrial protein transport and assembly?

It can recognize and bind mitochondrial proteins, and promote the correct folding and assembly of mitochondrial proteins by regulating the transport and assembly of other proteins.