"HSDL1" Related Products


Recombinant Human HSDL1 293 Cell Lysate

Cat.No.: HSDL1-5367HCL
Description: Antigen standard for hydroxysteroid dehydrogenase like 1 (HSDL1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HSDL1 hydroxysteroid dehydrogenase like 1 [ Homo sapiens ]
Official Symbol: HSDL1
Synonyms: HSDL1; hydroxysteroid dehydrogenase like 1; inactive hydroxysteroid dehydrogenase-like protein 1; SDR12C3; short chain dehydrogenase/reductase family 12C; member 3; steroid dehydrogenase-like protein; short chain dehydrogenase/reductase family 12C, member 3; MGC125994; MGC125995; MGC126032;
Gene ID: 83693
mRNA Refseq: NM_001146051
Protein Refseq: NP_001139523
UniProt ID: Q3SXM5
Chromosome Location: 16q24
Function: nucleotide binding; oxidoreductase activity;

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