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Recombinant Human HSD17B8 293 Cell Lysate

Cat.No. : HSD17B8-5371HCL
  • Specification
  • Gene Information
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Description : Antigen standard for hydroxysteroid (17-beta) dehydrogenase 8 (HSD17B8) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : HSD17B8 hydroxysteroid (17-beta) dehydrogenase 8 [ Homo sapiens ]
Official Symbol : HSD17B8
Synonyms : HSD17B8; hydroxysteroid (17-beta) dehydrogenase 8; FabG (beta ketoacyl [acyl carrier protein] reductase, E coli) like (E. coli) , FABGL; estradiol 17-beta-dehydrogenase 8; D6S2245E; H2 KE6; HKE6; KE6; RING2; SDR30C1; short chain dehydrogenase/reductase family 30C; member 1; ke-6; protein Ke6; 17-beta-HSD 8; estrogen 17-oxidoreductase; testosterone 17-beta-dehydrogenase 8; really interesting new gene 2 protein; 17-beta-hydroxysteroid dehydrogenase 8; 3-oxoacyl-[acyl-carrier-protein] reductase; short chain dehydrogenase/reductase family 30C, member 1; FABG; FABGL; H2-KE6; dJ1033B10.9;
Gene ID : 7923
mRNA Refseq : NM_014234
Protein Refseq : NP_055049
MIM : 601417
UniProt ID : Q92506
Chromosome Location : 6p21.3
Pathway : Metabolic pathways, organism-specific biosystem; Steroid hormone biosynthesis, organism-specific biosystem; Steroid hormone biosynthesis, conserved biosystem;
Function : 3-hydroxyacyl-CoA dehydrogenase activity; estradiol 17-beta-dehydrogenase activity; nucleotide binding; oxidoreductase activity; protein binding; testosterone 17-beta-dehydrogenase (NAD+) activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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