Recombinant Human HSD17B6 293 Cell Lysate


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Recombinant Human HSD17B6 293 Cell Lysate

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Cat.No. : HSD17B6-5372HCL
Description : Antigen standard for hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : HSD17B6 hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) [ Homo sapiens ]
Official Symbol : HSD17B6
Synonyms : HSD17B6; hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse); hydroxysteroid (17 beta) dehydrogenase 6; 17-beta-hydroxysteroid dehydrogenase type 6; 3(alpha >beta) hydroxysteroid epimerasel; 3 hydroxysteroid epimerase; HSE; NAD+ dependent 3 alpha hydroxysteroid dehydrogenase; oxidative 3 alpha hydroxysteroid dehydrogenase; oxidoreductase; retinol dehydrogenase; RODH; SDR9C6; short chain dehydrogenase/reductase family 9C; member 6; 17-beta-HSD6; 17-beta-HSD 6; 3-alpha->beta-HSE; 3-hydroxysteroid epimerase; beta-hydroxysteroid epimerase; 3(alpha->beta)-hydroxysteroid epimerase; beta)-hydroxysteroid epimerasel; oxidative 3-alpha hydroxysteroid dehydrogenase; oxidative 3-alpha-hydroxysteroid-dehydrogenase; short chain dehydrogenase/reductase family 9C, member 6; NAD+ -dependent 3 alpha-hydroxysteroid dehydrogenase 3-hydroxysteroid epimerase;
Gene ID : 8630
mRNA Refseq : NM_003725
Protein Refseq : NP_003716
MIM : 606623
UniProt ID : O14756
Chromosome Location : 12q13
Pathway : Metabolic pathways, organism-specific biosystem; Retinol metabolism, organism-specific biosystem; Retinol metabolism, conserved biosystem; Steroid hormone biosynthesis, organism-specific biosystem; Steroid hormone biosynthesis, conserved biosystem;
Function : catalytic activity; electron carrier activity; estradiol 17-beta-dehydrogenase activity; nucleotide binding; oxidoreductase activity; retinol dehydrogenase activity; testosterone 17-beta-dehydrogenase (NAD+) activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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