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Recombinant Human HSD11B2 293 Cell Lysate

Cat.No.: HSD11B2-5378HCL
Description: Antigen standard for hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HSD11B2 hydroxysteroid (11-beta) dehydrogenase 2 [ Homo sapiens ]
Official Symbol: HSD11B2
Synonyms: HSD11B2; hydroxysteroid (11-beta) dehydrogenase 2; corticosteroid 11-beta-dehydrogenase isozyme 2; SDR9C3; short chain dehydrogenase/reductase family 9C; member 3; 11-DH2; 11-beta-HSD2; -HSD11 type II; 11-beta-hydroxysteroid dehydrogenase type 2; 11-beta-hydroxysteroid dehydrogenase type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 9C member 3; AME; AME1; HSD2; HSD11K;
Gene ID: 3291
mRNA Refseq: NM_000196
Protein Refseq: NP_000187
MIM: 614232
UniProt ID: P80365
Chromosome Location: 16q22
Pathway: Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; C21-Steroid hormone biosynthesis, progesterone =>cortisol/cortisone, organism-specific biosystem; C21-Steroid hormone biosynthesis, progesterone => cortisol/cortisone, conserved biosystem; Glucocorticoid and
Function: 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity; NAD binding; oxidoreductase activity; steroid binding;

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