"HMGN3" Related Products

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Recombinant Human HMGN3 293 Cell Lysate

Cat.No.: HMGN3-5472HCL
Description: Antigen standard for high mobility group nucleosomal binding domain 3 (HMGN3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HMGN3 high mobility group nucleosomal binding domain 3 [ Homo sapiens ]
Official Symbol: HMGN3
Synonyms: HMGN3; high mobility group nucleosomal binding domain 3; thyroid hormone receptor interactor 7 , TRIP7; high mobility group nucleosome-binding domain-containing protein 3; TR-interacting protein 7; thyroid hormone receptor interacting protein 7; TRIP7; PNAS-24; PNAS-25; FLJ42187; DKFZp686E20226;
Gene ID: 9324
mRNA Refseq: NM_001201362
Protein Refseq: NP_001188291
MIM: 604502
UniProt ID: Q15651
Chromosome Location: 6q14.3
Function: DNA binding; chromatin binding; thyroid hormone receptor binding;

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