"HDAC9" Related Products

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Recombinant Human HDAC9 293 Cell Lysate

Cat.No.: HDAC9-5601HCL
Description: Antigen standard for histone deacetylase 9 (HDAC9), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: HDAC9 histone deacetylase 9 [ Homo sapiens ]
Official Symbol: HDAC9
Synonyms: HDAC9; histone deacetylase 9; HD7; HDAC; HDAC7B; KIAA0744; MITR; histone deacetylase 7B; histone deacetylase 4/5-related protein; MEF-2 interacting transcription repressor (MITR) protein; HD9; HD7b; HDRP; HDAC7; HDAC9B; HDAC9FL; DKFZp779K1053;
Gene ID: 9734
mRNA Refseq: NM_001204144
Protein Refseq: NP_001191073
MIM: 606543
UniProt ID: Q9UKV0
Chromosome Location: 7p21.1
Pathway: MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem; Signaling by NOTCH1, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; Signaling events mediated by HDAC Class II, organism-specific biosystem;
Function: NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; NOT histone deacetylase activity; histone deacetylase activity (H3-K16 specific); histone deacetylase binding; histone deacetylase binding; hydrolase activity; protein binding; protein deacetylase activity; protein kinase C binding; repressing transcription factor binding; repressing transcription factor binding; repressing transcription factor binding; transcription corepressor activity; transcription factor binding; transcription factor binding;

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