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Recombinant Human GYS2 293 Cell Lysate

Cat.No.: GYS2-5669HCL
Description: Antigen standard for glycogen synthase 2 (liver) (GYS2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GYS2 glycogen synthase 2 (liver) [ Homo sapiens ]
Official Symbol: GYS2
Synonyms: GYS2; glycogen synthase 2 (liver); glycogen [starch] synthase, liver;
Gene ID: 2998
mRNA Refseq: NM_021957
Protein Refseq: NP_068776
MIM: 138571
UniProt ID: P54840
Chromosome Location: 12p12.2-p11.2
Pathway: Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen synthesis, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Metabolism, organism-specific biosystem;
Function: glycogen (starch) synthase activity; glycogen (starch) synthase activity; protein homodimerization activity; transferase activity, transferring glycosyl groups;

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