"GTF2F2" Related Products

Expand
Expand

Recombinant Human GTF2F2 293 Cell Lysate

Cat.No.: GTF2F2-5699HCL
Description: Antigen standard for general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GTF2F2 general transcription factor IIF, polypeptide 2, 30kDa [ Homo sapiens ]
Official Symbol: GTF2F2
Synonyms: GTF2F2; general transcription factor IIF, polypeptide 2, 30kDa; general transcription factor IIF, polypeptide 1 (74kD subunit); general transcription factor IIF subunit 2; BTF4; RAP30; TFIIF; TFIIF-beta; ATP-dependent helicase GTF2F2; transcription initiation factor RAP30; general transcription factor IIF 30 kDa subunit; transcription initiation factor IIF subunit beta; TF2F2;
Gene ID: 2963
mRNA Refseq: NM_004128
Protein Refseq: NP_004119
MIM: 189969
UniProt ID: P13984
Chromosome Location: 13q14
Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Disease, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem;
Function: ATP binding; ATP-dependent helicase activity; DNA binding; helicase activity; hydrolase activity; nucleotide binding; protein binding;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.