Recombinant Human GNPDA2 293 Cell Lysate


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Recombinant Human GNPDA2 293 Cell Lysate

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Cat.No. : GNPDA2-5842HCL
Description : Antigen standard for glucosamine-6-phosphate deaminase 2 (GNPDA2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : GNPDA2 glucosamine-6-phosphate deaminase 2 [ Homo sapiens ]
Official Symbol : GNPDA2
Synonyms : GNPDA2; glucosamine-6-phosphate deaminase 2; glucosamine-6-phosphate isomerase 2; glucosamine 6 phosphate isomerase; SB52; GNPDA 2; 4921523I18Rik; glcN6P deaminase 2; glucosamine-6-phosphate isomerase SB52; putative glucosamine-6-phosphate isomerase; GNP2;
Gene ID : 132789
mRNA Refseq : NM_138335
Protein Refseq : NP_612208
MIM : 613222
UniProt ID : Q8TDQ7
Chromosome Location : 4p13
Pathway : Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Amino sugar and nucleotide sugar metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; N-acetylglucosamine degradation I, organism-specific biosystem; N-acetylglucosamine degradation II, organism-specific biosystem; UDP-N-acetyl-D-galactosamine biosynthesis II, organism-specific biosystem;
Function : glucosamine-6-phosphate deaminase activity; hydrolase activity; isomerase activity;

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