"GATA6" Related Products

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Recombinant Human GATA6 293 Cell Lysate

Cat.No.: GATA6-6009HCL
Description: Antigen standard for GATA binding protein 6 (GATA6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GATA6 GATA binding protein 6 [ Homo sapiens ]
Official Symbol: GATA6
Synonyms: GATA6; GATA binding protein 6; transcription factor GATA-6; GATA-binding factor 6; GATA-binding protein 6;
Gene ID: 2627
mRNA Refseq: NM_005257
Protein Refseq: NP_005248
MIM: 601656
UniProt ID: Q92908
Chromosome Location: 18q11-q12
Pathway: Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Heart Development, organism-specific biosystem; Hemostasis, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem;
Function: RNA polymerase II repressing transcription factor binding; chromatin binding; double-stranded DNA binding; metal ion binding; protein binding; protein kinase binding; sequence-specific DNA binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; zinc ion binding;

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