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Recombinant Human GANAB 293 Cell Lysate

Cat.No.: GANAB-6025HCL
Description: Antigen standard for glucosidase, alpha; neutral AB (GANAB), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GANAB glucosidase, alpha; neutral AB [ Homo sapiens ]
Official Symbol: GANAB
Synonyms: GANAB; glucosidase, alpha; neutral AB; neutral alpha-glucosidase AB; G2AN; GluII; KIAA0088; alpha-glucosidase 2; glucosidase II subunit alpha; alpha glucosidase II alpha subunit; GLUII;
Gene ID: 23193
mRNA Refseq: NM_198334
Protein Refseq: NP_938148
MIM: 104160
UniProt ID: Q14697
Chromosome Location: 11q12.3
Pathway: Asparagine N-linked glycosylation, organism-specific biosystem; Calnexin/calreticulin cycle, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan precursor trimming, organism-specific biosystem;
Function: carbohydrate binding; glucan 1,3-alpha-glucosidase activity; hydrolase activity, hydrolyzing O-glycosyl compounds;

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