"FOXH1" Related Products

Recombinant Human FOXH1 293 Cell Lysate

Cat.No.: FOXH1-6156HCL
Description: Antigen standard for forkhead box H1 (FOXH1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: FOXH1 forkhead box H1 [ Homo sapiens ]
Official Symbol: FOXH1
Synonyms: FOXH1; forkhead box H1; forkhead box protein H1; FAST1; fast-2; hFAST-1; TGF-beta/activin signal transducer; forkhead activin signal transducer 2; forkhead activin signal transducer-1; FAST-1;
Gene ID: 8928
mRNA Refseq: NM_003923
Protein Refseq: NP_003914
MIM: 603621
UniProt ID: O75593
Chromosome Location: 8q24
Pathway: Developmental Biology, organism-specific biosystem; Heart Development, organism-specific biosystem; Regulation of nuclear SMAD2/3 signaling, organism-specific biosystem; Signaling by NODAL, organism-specific biosystem; TGF Beta Signaling Pathway, organism-specific biosystem; TGF-beta Receptor Signaling Pathway, organism-specific biosystem;
Function: contributes_to DNA binding; DNA binding, bending; R-SMAD binding; double-stranded DNA binding; protein binding; protein domain specific binding; sequence-specific DNA binding; sequence-specific DNA binding; contributes_to sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription factor binding;

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