Recombinant Human FOXA2 293 Cell Lysate

• Cat.No.: FOXA2-6163HCL

Specification

• Description: Antigen standard for forkhead box A2 (FOXA2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: FOXA2 forkhead box A2 [ Homo sapiens ]
• Official Symbol: FOXA2
• Synonyms: FOXA2; forkhead box A2; hepatocyte nuclear factor 3, beta , HNF3B; hepatocyte nuclear factor 3-beta; HNF-3B; TCF-3B; HNF-3-beta; forkhead box protein A2; transcription factor 3B; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; HNF3B; TCF3B; MGC19807
• Gene ID: 3170
• mRNA Refseq: NM_021784
• Protein Refseq: NP_068556
• MIM: 600288
• UniProt ID: Q9Y261
• Chromosome Location: 20p11
• Pathway: Developmental Biology, organism-specific biosystem; FOXA transcription factor networks, organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Heart Development, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Maturity onset diabetes of the young, organism-specific biosystem
• Function: DNA binding; DNA binding, bending; RNA polymerase II core promoter proximal region sequence-specific DNA binding; SMAD binding; double-stranded DNA binding; protein domain specific binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription factor binding; transcription regulatory region DNA binding