"ETNK1" Related Products

Recombinant Human ETNK1 293 Cell Lysate

Cat.No.: ETNK1-6528HCL
Description: Antigen standard for ethanolamine kinase 1 (ETNK1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ETNK1 ethanolamine kinase 1 [ Homo sapiens ]
Official Symbol: ETNK1
Synonyms: ETNK1; ethanolamine kinase 1; EKI; EKI1; putative protein product of Nbla10396; EKI 1; Nbla10396;
Gene ID: 55500
mRNA Refseq: NM_001039481
Protein Refseq: NP_001034570
MIM: 609858
UniProt ID: Q9HBU6
Chromosome Location: 12p12.2
Pathway: Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Phosphatidylethanolamine (PE) biosynthesis, ethanolamine =>PE, organism-specific biosystem; Phosphatidylethanolamine (PE) biosynthesis, ethanolamine => PE, conserved biosystem;
Function: ATP binding; ethanolamine kinase activity; nucleotide binding;
Products Types ◆ Recombinant Protein

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