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Recombinant Human EPS15 293 Cell Lysate

Cat.No. : EPS15-6578HCL
  • Specification
  • Gene Information
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Description : Antigen standard for epidermal growth factor receptor pathway substrate 15 (EPS15), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : EPS15 epidermal growth factor receptor pathway substrate 15 [ Homo sapiens ]
Official Symbol : EPS15
Synonyms : EPS15; epidermal growth factor receptor pathway substrate 15; epidermal growth factor receptor substrate 15; AF 1P; MLLT5; protein AF-1p; ALL1 fused gene from chromosome 1; AF1P; AF-1P;
Gene ID : 2060
mRNA Refseq : NM_001159969
Protein Refseq : NP_001153441
MIM : 600051
UniProt ID : P42566
Chromosome Location : 1p32
Pathway : Disease, organism-specific biosystem; EGFR downregulation, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Internalization of ErbB1, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem;
Function : SH3 domain binding; calcium ion binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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