Recombinant Human EOMES 293 Cell Lysate

• Cat.No.: EOMES-6589HCL

Specification

• Description: Antigen standard for eomesodermin homolog (Xenopus laevis) (EOMES) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: EOMES eomesodermin [ Homo sapiens ]
• Official Symbol: EOMES
• Synonyms: EOMES; eomesodermin; eomesodermin (Xenopus laevis) homolog; eomesodermin homolog; T box brain2; TBR2; TBR-2; T-brain-2; T-box brain2; t box, brain, 2; T-box brain protein 2
• Gene ID: 8320
• mRNA Refseq: NM_005442
• Protein Refseq: NP_005433
• MIM: 604615
• UniProt ID: O95936
• Chromosome Location: 3p24.1
• Pathway: Downstream signaling in naive CD8+ T cells, organism-specific biosystem; IL12-mediated signaling events, organism-specific biosystem
• Function: DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity

Reviews

07/18/2019

The storage conditions and experimental environment of this protein are simple and convenient without specific conditions.

04/10/2019

Less polluting and minimizes waste.

03/31/2019

You can see that this protein is produced with great care for purity and activity.

Q&As

What is the role of EOMES in lung cancer?

EOMES can affect the production, progression and prognosis of lung cancer by regulating CTLs function and immune surveillance cell types and functions.

What is the role of EOMES in T lymphocyte differentiation and function?

EOMES plays a key role in the differentiation and function of CD8+ T cells, particularly in the formation and stability maintenance of effector memory T cells and cytotoxic T lymphocytes (CTLs).

How can immune-related diseases be studied by EOMES gene?

The molecular mechanisms of EOMES genes can be studied by analyzing the association between the EOMES gene and immune-related diseases such as autoimmune diseases, infectious diseases, etc.

Is there a link between EOMES and certain lymphomas?

Yes, EOMES is strongly associated with the occurrence and progression of a variety of lymphoid tumors (such as Hodgkin lymphoma, T-cell lymphoma, etc.).

How to study the role of EOMES in the immune system?

Mouse gene knockout technology, small RNA interference technology, etc. can be used to study the role of EOMES in the immune system.

How about the role of EOMES in tumor immunotherapy?

EOMES can enhance the reactivity of T lymphocytes in tumor immunotherapy, thereby enhancing the killing ability and therapeutic effect of tumor cells.