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Recombinant Human ENTPD6 293 Cell Lysate

Cat.No.: ENTPD6-6591HCL
Description: Antigen standard for ectonucleoside triphosphate diphosphohydrolase 6 (putative function) (ENTPD6), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ENTPD6 ectonucleoside triphosphate diphosphohydrolase 6 (putative) [ Homo sapiens ]
Official Symbol: ENTPD6
Synonyms: ENTPD6; ectonucleoside triphosphate diphosphohydrolase 6 (putative); CD39L2, IL6ST2, interleukin 6 signal transducer 2; ectonucleoside triphosphate diphosphohydrolase 6; dJ738P15.3; NTPDase 6; CD39-like 2; CD39 antigen-like 2; HCV-E2 binding protein 1; interleukin 6 signal transducer-2; ectonucleoside triphosphate diphosphohydrolase 6 (putative function); CD39L2; IL6ST2; IL-6SAG; NTPDase-6; FLJ36711; DKFZp781G2277; DKFZp781K21102;
Gene ID: 955
mRNA Refseq: NM_001114089
Protein Refseq: NP_001107561
MIM: 603160
UniProt ID: O75354
Chromosome Location: 20p11.21
Pathway: Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Pyrimidine metabolism, organism-specific biosystem; Pyrimidine metabolism, conserved biosystem;
Function: guanosine-5-triphosphate,3-diphosphate diphosphatase activity; hydrolase activity; nucleoside-triphosphatase activity; uridine-diphosphatase activity;

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