"EMID1" Related Products

Recombinant Human EMID1 293 Cell Lysate

Cat.No.: EMID1-6610HCL
Description: Antigen standard for EMI domain containing 1 (EMID1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: EMID1 EMI domain containing 1 [ Homo sapiens ]
Official Symbol: EMID1
Synonyms: EMID1; EMI domain containing 1; EMI domain-containing protein 1; EMI5; emilin and multimerin domain containing protein 1; EMU1; hEmu1; putative emu1; emilin and multimerin domain-containing protein 1; emilin and multimerin-domain containing protein 1; MGC50657;
Gene ID: 129080
mRNA Refseq: NM_133455
Protein Refseq: NP_597712
MIM: 608926
UniProt ID: Q96A84
Chromosome Location: 22q12.2

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