Recombinant Human EIF4H 293 Cell Lysate

• Cat.No.: EIF4H-6643HCL

Specification

• Description: Antigen standard for eukaryotic translation initiation factor 4H (EIF4H), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: EIF4H eukaryotic translation initiation factor 4H [ Homo sapiens ]
• Official Symbol: EIF4H
• Synonyms: EIF4H; eukaryotic translation initiation factor 4H; WBSCR1, Williams Beuren syndrome chromosome region 1; KIAA0038; WSCR1; Williams-Beuren syndrome chromosome region 1; WBSCR1; eIF-4H
• Gene ID: 7458
• mRNA Refseq: NM_022170
• Protein Refseq: NP_071496
• MIM: 603431
• UniProt ID: Q15056
• Chromosome Location: 7q11.23
• Pathway: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem; Gene Expression, organism-specific biosystem; L13a-mediated translational silencing of Ceruloplasmin expression, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem
• Function: RNA binding; nucleotide binding; protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity