Recombinant Human DDX5 293 Cell Lysate

• Cat.No.: DDX5-7004HCL

Specification

• Description: Antigen standard for DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DDX5 DEAD (Asp-Glu-Ala-Asp) box helicase 5 [ Homo sapiens ]
• Official Symbol: DDX5
• Synonyms: DDX5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp Glu Ala Asp) box polypeptide 5 , DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) , G17P1, HLR1; probable ATP-dependent RNA helicase DDX5; p68; DEAD box-5; RNA helicase p68; DEAD box protein 5; ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); HLR1; G17P1; HUMP68; DKFZp434E109; DKFZp686J01190
• Gene ID: 1655
• mRNA Refseq: NM_004396
• Protein Refseq: NP_004387
• MIM: 180630
• UniProt ID: P17844
• Chromosome Location: 17q21
• Pathway: Direct p53 effectors, organism-specific biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: ATP binding; ATP-dependent helicase activity; RNA helicase activity; RNA helicase activity; androgen receptor binding; estrogen receptor binding; hydrolase activity; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; nucleotide binding; pre-mRNA binding; protein binding; transcription coactivator activity