"DACT1" Related Products

Recombinant Human DACT1 293 Cell Lysate

Cat.No.: DACT1-7084HCL
Description: Antigen standard for dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: DACT1 dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) [ Homo sapiens ]
Official Symbol: DACT1
Synonyms: DACT1; dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis); dapper homolog 1, antagonist of beta catenin (xenopus); dapper homolog 1; DAPPER; DAPPER1; FRODO; HDPR1; THYEX3; heptacellular carcinoma novel gene 3; hepatocellular carcinoma novel gene 3 protein; DPR1;
Gene ID: 51339
mRNA Refseq: NM_001079520
Protein Refseq: NP_001072988
MIM: 607861
UniProt ID: Q9NYF0
Chromosome Location: 14q22.3

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