"CSDE1" Related Products

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Recombinant Human CSDE1 293 Cell Lysate

Cat.No.: CSDE1-7249HCL
Description: Antigen standard for cold shock domain containing E1, RNA-binding (CSDE1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CSDE1 cold shock domain containing E1, RNA-binding [ Homo sapiens ]
Official Symbol: CSDE1
Synonyms: CSDE1; cold shock domain containing E1, RNA-binding; cold shock domain-containing protein E1; D1S155E; UNR; upstream of NRAS; NRAS-related; N-ras upstream gene protein; FLJ26882; RP5-1000E10.3; DKFZp779B0247; DKFZp779J1455;
Gene ID: 7812
mRNA Refseq: NM_001007553
Protein Refseq: NP_001007554
MIM: 191510
UniProt ID: O75534
Chromosome Location: 1p13.2
Pathway: Validated targets of C-MYC transcriptional repression, organism-specific biosystem;
Function: DNA binding; RNA binding;

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