"CLCN7" Related Products

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Recombinant Human CLCN7 293 Cell Lysate

Cat.No.: CLCN7-7472HCL
Description: Antigen standard for chloride channel 7 (CLCN7), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CLCN7 chloride channel, voltage-sensitive 7 [ Homo sapiens ]
Official Symbol: CLCN7
Synonyms: CLCN7; chloride channel, voltage-sensitive 7; chloride channel 7; H(+)/Cl(-) exchange transporter 7; CLC 7; ClC 7; CLC7; OPTA2; PPP1R63; protein phosphatase 1; regulatory subunit 63; chloride channel protein 7; protein phosphatase 1, regulatory subunit 63; CLC-7; OPTB4; FLJ26686; FLJ39644; FLJ46423;
Gene ID: 1186
mRNA Refseq: NM_001114331
Protein Refseq: NP_001107803
MIM: 602727
UniProt ID: P51798
Chromosome Location: 16p13
Function: ATP binding; antiporter activity; chloride channel activity; ion channel activity; nucleotide binding; voltage-gated chloride channel activity;

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