"CHST12" Related Products

Expand

Recombinant Human CHST12 293 Cell Lysate

Cat.No.: CHST12-7507HCL
Description: Antigen standard for carbohydrate (chondroitin 4) sulfotransferase 12 (CHST12) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CHST12 carbohydrate (chondroitin 4) sulfotransferase 12 [ Homo sapiens ]
Official Symbol: CHST12
Synonyms: CHST12; carbohydrate (chondroitin 4) sulfotransferase 12; carbohydrate sulfotransferase 12; C4S 2; C4ST2; sulfotransferase Hlo; chondroitin 4-sulfotransferase 2; chondroitin 4-O-sulfotransferase 2; C4S-2; C4ST-2;
Gene ID: 55501
mRNA Refseq: NM_001243794
Protein Refseq: NP_001230723
MIM: 610129
UniProt ID: Q9NRB3
Chromosome Location: 7p22
Pathway: Glycosaminoglycan biosynthesis - chondroitin sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - chondroitin sulfate, conserved biosystem; Sulfur metabolism, organism-specific biosystem; Sulfur metabolism, conserved biosystem; chondroitin sulfate biosynthesis, organism-specific biosystem; chondroitin sulfate biosynthesis, conserved biosystem; chondroitin sulfate biosynthesis (late stages), organism-specific biosystem;
Function: 3-phosphoadenosine 5-phosphosulfate binding; chondroitin 4-sulfotransferase activity; sulfotransferase activity; transferase activity;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.