"CDK6" Related Products

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Recombinant Human CDK6 293 Cell Lysate

Cat.No.: CDK6-7622HCL
Description: Antigen standard for cyclin-dependent kinase 6 (CDK6), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CDK6 cyclin-dependent kinase 6 [ Homo sapiens ]
Official Symbol: CDK6
Synonyms: CDK6; cyclin-dependent kinase 6; PLSTIRE; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE; MGC59692;
Gene ID: 1021
mRNA Refseq: NM_001145306
Protein Refseq: NP_001138778
MIM: 603368
UniProt ID: Q00534
Chromosome Location: 7q21-q22
Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem;
Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding;

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