"PRIMPOL" Related Products

Recombinant Human CCDC111 293 Cell Lysate

Cat.No.: PRIMPOL-7789HCL
Description: Antigen standard for coiled-coil domain containing 111 (CCDC111) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRIMPOL?primase and polymerase (DNA-directed) [?Homo sapiens?(human) ]
Official Symbol: PRIMPOL
Synonyms: PRIMPOL; MYP22; CCDC111; primase and polymerase (DNA-directed); DNA-directed primase/polymerase protein; hPrimpol1; coiled-coil domain containing 111; coiled-coil domain-containing protein 111; NP_689896.1; EC 2.7.7.-
Gene ID: 201973
mRNA Refseq: NM_152683
Protein Refseq: NP_689896
MIM: 615421
UniProt ID: Q96LW4
Chromosome Location: 4q35.1
Function: DNA primase activity; DNA-directed DNA polymerase activity; chromatin binding; manganese ion binding

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