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Recombinant Human CBLB 293 Cell Lysate

Cat.No.: CBLB-7814HCL
Description: Antigen standard for Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CBLB Cbl proto-oncogene, E3 ubiquitin protein ligase B [ Homo sapiens ]
Official Symbol: CBLB
Synonyms: CBLB; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cas Br M (murine) ecotropic retroviral transforming sequence b , Cas Br M (murine) ectropic retroviral transforming sequence b; E3 ubiquitin-protein ligase CBL-B; Cbl b; RNF56; RING finger protein 56; SH3-binding protein CBL-B; signal transduction protein CBL-B; casitas B-lineage lymphoma proto-oncogene b; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Cas-Br-M (murine) ecotropic retroviral transforming sequence b; Cbl-b; Nbla00127; FLJ36865; FLJ41152; DKFZp779A0729; DKFZp779F1443; DKFZp686J10223;
Gene ID: 868
mRNA Refseq: NM_170662
Protein Refseq: NP_733762
MIM: 604491
UniProt ID: Q13191
Chromosome Location: 3q
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem;
Function: calcium ion binding; ligase activity; phosphotyrosine binding; protein binding; protein kinase binding; signal transducer activity; ubiquitin-protein ligase activity; zinc ion binding;

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