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Recombinant Human CARM1 293 Cell Lysate

Cat.No.: CARM1-7846HCL
Description: Antigen standard for coactivator-associated arginine methyltransferase 1 (CARM1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CARM1 coactivator-associated arginine methyltransferase 1 [ Homo sapiens ]
Official Symbol: CARM1
Synonyms: CARM1; coactivator-associated arginine methyltransferase 1; histone-arginine methyltransferase CARM1; PRMT4; protein arginine N-methyltransferase 4;
Gene ID: 10498
mRNA Refseq: NM_199141
Protein Refseq: NP_954592
MIM: 603934
UniProt ID: Q86X55
Chromosome Location: 19p13.2
Pathway: BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem;
Function: beta-catenin binding; histone acetyl-lysine binding; histone methyltransferase activity; histone methyltransferase activity (H3-R17 specific); ligand-dependent nuclear receptor transcription coactivator activity; protein homodimerization activity; protein methyltransferase activity; protein-arginine N-methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; transcription coactivator activity; transcription regulatory region DNA binding; transferase activity;

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