Recombinant Human CARD11 293 Cell Lysate

• Cat.No.: CARD11-7850HCL

Specification

• Description: Antigen standard for caspase recruitment domain family, member 11 (CARD11) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CARD11 caspase recruitment domain family, member 11 [ Homo sapiens ]
• Official Symbol: CARD11
• Synonyms: CARD11; caspase recruitment domain family, member 11; caspase recruitment domain-containing protein 11; bcl10 interacting maguk protein 3; BIMP3; card maguk protein 1; CARMA1; carma 1; card-maguk protein 1; CARD-containing MAGUK protein 1; bcl10-interacting maguk protein 3; MGC133069
• Gene ID: 84433
• mRNA Refseq: NM_032415
• Protein Refseq: NP_115791
• MIM: 607210
• UniProt ID: Q9BXL7
• Chromosome Location: 7p22
• Pathway: Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; BCR signaling pathway, organism-specific biosystem; Downstream Signaling Events Of B Cell Receptor (BCR), organism-specific biosystem
• Function: CARD domain binding; guanylate kinase activity; protein binding