"CAMK2D" Related Products

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Recombinant Human CAMK2D 293 Cell Lysate

Cat.No.: CAMK2D-7878HCL
Description: Antigen standard for calcium/calmodulin-dependent protein kinase II delta (CAMK2D), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CAMK2D calcium/calmodulin-dependent protein kinase II delta [ Homo sapiens ]
Official Symbol: CAMK2D
Synonyms: CAMK2D; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin dependent protein kinase (CaM kinase) II delta , CAMKD; calcium/calmodulin-dependent protein kinase type II subunit delta; CaMK-II delta subunit; caMK-II subunit delta; CaM-kinase II delta chain; CaM kinase II delta subunit; caM kinase II subunit delta; calcium/calmodulin-dependent protein kinase type II delta chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; CAMKD; MGC44911; DKFZp686I2288; DKFZp686G23119;
Gene ID: 817
mRNA Refseq: NM_172115
Protein Refseq: NP_742113
MIM: 607708
UniProt ID: Q13557
Chromosome Location: 4q26
Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; CREB phosphorylation through the activation of CaMKII, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem;
Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; nucleotide binding;

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