Recombinant Human CA3 293 Cell Lysate
Cat.No. : | CA3-7914HCL |
- Specification
- Gene Information
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Description : | Antigen standard for carbonic anhydrase III, muscle specific (CA3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | CA3 carbonic anhydrase III, muscle specific [ Homo sapiens ] |
Official Symbol : | CA3 |
Synonyms : | CA3; carbonic anhydrase III, muscle specific; carbonic anhydrase 3; CAIII; Car3; CA-III; carbonate dehydratase III; FLJ36434; |
Gene ID : | 761 |
mRNA Refseq : | NM_005181 |
Protein Refseq : | NP_005172 |
MIM : | 114750 |
UniProt ID : | P07451 |
Chromosome Location : | 8q21.2 |
Function : | carbonate dehydratase activity; lyase activity; metal ion binding; nickel cation binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
CA3-0239H | Recombinant Human CA3 Protein, GST-Tagged | +Inquiry |
CA3-717R | Recombinant Rat CA3 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA3-1051H | Recombinant Human CA3 Protein (M1-K260), His/Strep tagged | +Inquiry |
Car3-766M | Recombinant Mouse Car3 Protein, MYC/DDK-tagged | +Inquiry |
CA3-480H | Recombinant Human CA3 Protein, His (Fc)-Avi-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionCA3 is primarily found in skeletal muscles.
Post-translational modifications, like phosphorylation or glycosylation, can be identified using mass spectrometry.
Specific inhibitors for CA3 are yet to be fully elucidated and would be determined using enzymatic assays.
CA3 plays a role in CO2 and bicarbonate equilibrium, crucial for metabolic pathways like glycolysis and the citric acid cycle.
While CA3 has a similar mechanism, its activity and affinity might differ from other isoforms, requiring comparative studies.
CA3 dysregulation might be linked to muscle diseases or metabolic disorders, requiring further research.
CA3 catalyzes the reversible hydration of carbon dioxide, aiding in pH regulation and CO2 transport.
Customer Reviews (3)
Write a reviewOutstanding protein sequencing capabilities, unrivaled accuracy.
Precise purification techniques, yield exceptional samples.
Efficient sample handling, optimizes our laboratory workflow.
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