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Recombinant Human ACAT2, T7-tagged

Cat. No. : ACAT2-29H
Product Overview : Recombinant Human Acetyl-Coenzyme A acetyltransferase 2 produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids 1-397 and having a total molecular mass of 41.3 kda. ACAT2 contains T7 tag at N-terminus. ACAT-2 is purified by proprietary chromatographic techniques.
Description : Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3 prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
Source : Escherichia Coli.
Physical Appearance : Sterile Filtered clear solution (0.77 mg/ml).
Purity : Greater than 95.0% as determined by SDS-PAGE.
Formulation : Acetyl-CoA acetyltransferase cytosolic at a concentration of 0.1 mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
Applications : • ELISA • MS • Inhibition Assays • Western Blotting.
Stability : ACAT2 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name : ACAT2 acetyl-Coenzyme A acetyltransferase 2 [ Homo sapiens ]
Synonyms : ACAT2; acetyl-Coenzyme A acetyltransferase 2;Acetyl-CoA acetyltransferase, cytosolic;Cytosolic acetoacetyl-CoA thiolase; Acetyl-CoA transferase-like protein; EC 2.3.1.9; OTTHUMP00000017527; Cytosolic acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 2; acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)
Gene ID : 39
mRNA Refseq : NM_005891
Protein Refseq : NP_005882
UniProt ID : Q9BWD1
Chromosome Location : 6q25.3
MIM : 100678
Pathway : Benzoate degradation via CoA ligation; Butanoate metabolism; Fatty acid metabolism; Lysine degradation; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism; Synthesis and degradation of ketone bodies; Terpenoid backbone biosynthesis; Tryptophan metabolism; Valine, leucine and isoleucine degradation
Function : acetyl-CoA C-acetyltransferase activity; acyltransferase activity; protein binding; transferase activity

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