Recombinant Human ACAT2, T7-tagged

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Recombinant Human ACAT2, T7-tagged

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Cat. No. : ACAT2-29H
Product Overview : Recombinant Human Acetyl-Coenzyme A acetyltransferase 2 produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids 1-397 and having a total molecular mass of 41.3 kda. ACAT2 contains T7 tag at N-terminus. ACAT-2 is purified by proprietary chromatographic techniques.
Description : Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3 prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
Source : Escherichia Coli.
Physical Appearance : Sterile Filtered clear solution (0.77 mg/ml).
Purity : Greater than 95.0% as determined by SDS-PAGE.
Formulation : Acetyl-CoA acetyltransferase cytosolic at a concentration of 0.1 mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
Applications : • ELISA • MS • Inhibition Assays • Western Blotting.
Stability : ACAT2 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name : ACAT2 acetyl-Coenzyme A acetyltransferase 2 [ Homo sapiens ]
Synonyms : ACAT2; acetyl-Coenzyme A acetyltransferase 2;Acetyl-CoA acetyltransferase, cytosolic;Cytosolic acetoacetyl-CoA thiolase; Acetyl-CoA transferase-like protein; EC 2.3.1.9; OTTHUMP00000017527; Cytosolic acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 2; acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)
Gene ID : 39
mRNA Refseq : NM_005891
Protein Refseq : NP_005882
UniProt ID : Q9BWD1
Chromosome Location : 6q25.3
MIM : 100678
Pathway : Benzoate degradation via CoA ligation; Butanoate metabolism; Fatty acid metabolism; Lysine degradation; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism; Synthesis and degradation of ketone bodies; Terpenoid backbone biosynthesis; Tryptophan metabolism; Valine, leucine and isoleucine degradation
Function : acetyl-CoA C-acetyltransferase activity; acyltransferase activity; protein binding; transferase activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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