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Recombinant Human ACAT2, T7-tagged

Cat. No.: ACAT2-29H
Product Overview: Recombinant Human Acetyl-Coenzyme A acetyltransferase 2 produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids 1-397 and having a total molecular mass of 41.3 kda. ACAT2 contains T7 tag at N-terminus. ACAT-2 is purified by proprietary chromatographic techniques.
Description: Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3 prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered clear solution (0.77 mg/ml).
Purity: Greater than 95.0% as determined by SDS-PAGE.
Formulation: Acetyl-CoA acetyltransferase cytosolic at a concentration of 0.1 mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
Applications: • ELISA • MS • Inhibition Assays • Western Blotting.
Stability: ACAT2 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: ACAT2 acetyl-Coenzyme A acetyltransferase 2 [ Homo sapiens ]
Synonyms: ACAT2; acetyl-Coenzyme A acetyltransferase 2;Acetyl-CoA acetyltransferase, cytosolic;Cytosolic acetoacetyl-CoA thiolase; Acetyl-CoA transferase-like protein; EC 2.3.1.9; OTTHUMP00000017527; Cytosolic acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 2; acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)
Gene ID: 39
mRNA Refseq: NM_005891
Protein Refseq: NP_005882
UniProt ID: Q9BWD1
Chromosome Location: 6q25.3
MIM: 100678
Pathway: Benzoate degradation via CoA ligation; Butanoate metabolism; Fatty acid metabolism; Lysine degradation; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism; Synthesis and degradation of ketone bodies; Terpenoid backbone biosynthesis; Tryptophan metabolism; Valine, leucine and isoleucine degradation
Function: acetyl-CoA C-acetyltransferase activity; acyltransferase activity; protein binding; transferase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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