||Recombinant Human Acetyl-Coenzyme A acetyltransferase 2 produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids 1-397 and having a total molecular mass of 41.3 kda. ACAT2 contains T7 tag at N-terminus. ACAT-2 is purified by proprietary chromatographic techniques.
||Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3 prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
||Sterile Filtered clear solution (0.77 mg/ml).
||Greater than 95.0% as determined by SDS-PAGE.
||Acetyl-CoA acetyltransferase cytosolic at a concentration of 0.1 mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
||• ELISA • MS • Inhibition Assays • Western Blotting.
||ACAT2 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.