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Recombinant Human ATP2A2 293 Cell Lysate

Cat.No.: ATP2A2-8609HCL
Description: Antigen standard for ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (ATP2A2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ATP2A2?ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 [?Homo sapiens?(human) ]
Official Symbol: ATP2A2
Synonyms: ATP2A2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; DD; DAR; ATP2B; SERCA2; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; calcium pump 2; SR Ca(2+)-ATPase 2; cardiac Ca2+ ATPase; endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; NP_001672.1; EC 3.6.3.8; NP_733765.1
Gene ID: 488
mRNA Refseq: NM_170665
Protein Refseq: NP_733765
MIM: 108740
UniProt ID: P16615
Chromosome Location: 12q24.11
Pathway: Adrenergic signaling in cardiomyocytes, organism-specific biosystem; Alzheimer's disease, conserved biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Cardiac muscle contraction, organism-specific biosystem
Function: ATP binding; calcium-transporting ATPase activity; calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential

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