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Recombinant Human ASAP1 293 Cell Lysate

Cat.No.: ASAP1-8668HCL
Description: Antigen standard for ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ASAP1 ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 [ Homo sapiens ]
Official Symbol: ASAP1
Synonyms: ASAP1; ArfGAP with SH3 domain, ankyrin repeat and PH domain 1; DDEF1, development and differentiation enhancing factor 1; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; centaurin; beta 4; CENTB4; KIAA1249; PAP; ZG14P; DEF-1; centaurin, beta 4; PIP2-dependent ARF1 GAP; ARF GTPase-activating protein 1; development and differentiation-enhancing factor 1; ADP-ribosylation factor-directed GTPase-activating protein 1; 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; PAG2; AMAP1; DDEF1;
Gene ID: 50807
mRNA Refseq: NM_001247996
Protein Refseq: NP_001234925
MIM: 605953
UniProt ID: Q9ULH1
Chromosome Location: 8q24.1-q24.2
Pathway: Arf1 pathway, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Signaling events mediated by focal adhesion kinase, organism-specific biosystem;
Function: ARF GTPase activator activity; SH3 domain binding; cytoskeletal adaptor activity; metal ion binding; protein binding; zinc ion binding;

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