Recombinant Human ANAPC11 293 Cell Lysate

• Cat.No.: ANAPC11-8870HCL

Specification

• Description: Antigen standard for anaphase promoting complex subunit 11 (ANAPC11), transcript variant 5 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ANAPC11 anaphase promoting complex subunit 11 [ Homo sapiens ]
• Official Symbol: ANAPC11
• Synonyms: ANAPC11; anaphase promoting complex subunit 11; anaphase promoting complex subunit 11 (yeast APC11 homolog); anaphase-promoting complex subunit 11; APC11; Apc11p; HSPC214; MGC882; cyclosome subunit 11; APC11 anaphase promoting complex subunit 11 homolog; hepatocellular carcinoma-associated RING finger protein
• Gene ID: 51529
• mRNA Refseq: NM_001002247
• Protein Refseq: NP_001002247
• MIM: 614534
• UniProt ID: Q9NYG5
• Chromosome Location: 17q25.3
• Pathway: APC/C complex, organism-specific biosystem; APC/C complex, conserved biosystem; APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem
• Function: metal ion binding; contributes_to ubiquitin-protein ligase activity; zinc ion binding