Recombinant Human ALPK1 293 Cell Lysate
Cat.No. : | ALPK1-8894HCL |
- Specification
- Gene Information
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Description : | Antigen standard for alpha-kinase 1 (ALPK1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ALPK1 alpha-kinase 1 [ Homo sapiens ] |
Official Symbol : | ALPK1 |
Synonyms : | ALPK1; alpha-kinase 1; alpha-protein kinase 1; FLJ22670; KIAA1527; Lak; lymphocyte alpha kinase; chromosome 4 kinase; lymphocyte alpha-kinase; lymphocyte alpha-protein kinase; LAK; 8430410J10Rik; |
Gene ID : | 80216 |
mRNA Refseq : | NM_001102406 |
Protein Refseq : | NP_001095876 |
MIM : | 607347 |
UniProt ID : | Q96QP1 |
Chromosome Location : | 4q26 |
Function : | ATP binding; kinase activity; protein serine/threonine kinase activity; |
Products Types
◆ Recombinant Protein | ||
ALPK1-327H | Recombinant Human ALPK1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Alpk1-1605M | Recombinant Mouse Alpk1 Protein, Myc/DDK-tagged | +Inquiry |
ALPK1-001H | Recombinant Human ALPK1 Protein, Myc/DDK-tagged | +Inquiry |
ALPK1-5985H | Recombinant Human ALPK1 protein, His&Myc-tagged | +Inquiry |
ALPK1-3097H | Recombinant Human ALPK1 protein, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionYes, ALPK1 kinase activity can be modulated by specific small molecule inhibitors that target its ATP-binding site.
ALPK1 influences intracellular signaling cascades such as MAPK and AKT pathways and can modulate the expression of specific target genes.
ALPK1 knockout or overexpression can lead to altered cell morphology, proliferation rates, and cell cycle progression.
ALPK1 is primarily localized in the cytoplasm of human cells.
ALPK1 has been associated with both pro-apoptotic and anti-apoptotic signaling pathways, suggesting a complex role in cellular survival.
Yes, the expression of ALPK1 can be upregulated or downregulated in response to various stress stimuli.
ALPK1 is implicated in promoting cellular proliferation and growth.
ALPK1 has been implicated in regulating cellular migration and invasion in certain cell types.
Phosphorylation is a known post-translational modification that can regulate ALPK1 activity.
Several interacting proteins and signaling pathways have been identified, including protein kinases, phosphatases, and cytoskeletal regulators.
Customer Reviews (3)
Write a reviewHighly effective fusion protein detection.
Exceptional protein-protein interaction analysis.
Specific target detection in immunohistochemistry.
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