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Recombinant Human ALPK1 293 Cell Lysate

Cat.No. : ALPK1-8894HCL
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  • Gene Information
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Description : Antigen standard for alpha-kinase 1 (ALPK1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ALPK1 alpha-kinase 1 [ Homo sapiens ]
Official Symbol : ALPK1
Synonyms : ALPK1; alpha-kinase 1; alpha-protein kinase 1; FLJ22670; KIAA1527; Lak; lymphocyte alpha kinase; chromosome 4 kinase; lymphocyte alpha-kinase; lymphocyte alpha-protein kinase; LAK; 8430410J10Rik;
Gene ID : 80216
mRNA Refseq : NM_001102406
Protein Refseq : NP_001095876
MIM : 607347
UniProt ID : Q96QP1
Chromosome Location : 4q26
Function : ATP binding; kinase activity; protein serine/threonine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can ALPK1 kinase activity be modulated by specific small molecules or inhibitors? 08/29/2022

Yes, ALPK1 kinase activity can be modulated by specific small molecule inhibitors that target its ATP-binding site.

How does ALPK1 influence intracellular signaling cascades or gene expression patterns? 10/26/2021

ALPK1 influences intracellular signaling cascades such as MAPK and AKT pathways and can modulate the expression of specific target genes.

What is the impact of ALPK1 knockout or overexpression on cellular phenotypes? 08/18/2021

ALPK1 knockout or overexpression can lead to altered cell morphology, proliferation rates, and cell cycle progression.

What is the subcellular localization of ALPK1 in human cells? 08/27/2020

ALPK1 is primarily localized in the cytoplasm of human cells.

Is ALPK1 involved in the regulation of cellular apoptosis or survival pathways? 02/26/2020

ALPK1 has been associated with both pro-apoptotic and anti-apoptotic signaling pathways, suggesting a complex role in cellular survival.

Does ALPK1 expression change under specific cellular stress conditions? 09/10/2019

Yes, the expression of ALPK1 can be upregulated or downregulated in response to various stress stimuli.

How does ALPK1 contribute to cellular proliferation and growth? 09/05/2019

ALPK1 is implicated in promoting cellular proliferation and growth.

Does ALPK1 play a role in cellular migration or invasion processes? 04/08/2019

ALPK1 has been implicated in regulating cellular migration and invasion in certain cell types.

Are there any specific post-translational modifications that regulate ALPK1 activity? 07/09/2018

Phosphorylation is a known post-translational modification that can regulate ALPK1 activity.

Are there any known interacting proteins or signaling pathways associated with ALPK1? 08/02/2017

Several interacting proteins and signaling pathways have been identified, including protein kinases, phosphatases, and cytoskeletal regulators.

Customer Reviews (3)

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Reviews
03/26/2022

    Highly effective fusion protein detection.

    06/16/2019

      Exceptional protein-protein interaction analysis.

      02/09/2017

        Specific target detection in immunohistochemistry.

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