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Recombinant Human ADPGK 293 Cell Lysate

Cat.No. : ADPGK-9004HCL
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  • Gene Information
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Description : Antigen standard for ADP-dependent glucokinase (ADPGK), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ADPGK ADP-dependent glucokinase [ Homo sapiens ]
Official Symbol : ADPGK
Synonyms : ADPGK; ADP-dependent glucokinase; ADP GK; DKFZp434B195; rbBP-35; ATP-dependent glucokinase; ADP-GK; 2610017G09Rik;
Gene ID : 83440
mRNA Refseq : NM_031284
Protein Refseq : NP_112574
MIM : 611861
UniProt ID : Q9BRR6
Chromosome Location : 15q24.1
Pathway : Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Metabolic pathways, organism-specific biosystem;
Function : ADP-specific glucokinase activity; metal ion binding; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (13)

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Can mutations in the ADPGK gene cause disease? 01/30/2023

Currently, there is limited evidence to suggest that mutations in the ADPGK gene itself directly cause disease. However, some studies have identified single nucleotide polymorphisms (SNPs) in ADPGK that may be associated with increased risk of certain disease states, such as Alzheimer's disease and type 2 diabetes.

What are the challenges in studying Adpgk? 07/06/2022

One of the main challenges in studying Adpgk is the lack of knowledge about its cellular functions and regulatory mechanisms. Additionally, because Adpgk is a relatively understudied gene, there is limited information available about its interactions with other proteins and cellular processes. More research is needed to fully understand the role of Adpgk in health and disease.

How is Adpgk studied in the laboratory? 06/24/2021

Researchers can study Adpgk in the laboratory using a variety of techniques, such as enzyme assays, western blotting, and immunohistochemistry. They may also use genetic and molecular biology tools to manipulate Adpgk expression or function in cells or animal models.

Is there any ongoing research on Adpgk? 12/13/2019

Yes, there is ongoing research on Adpgk in a variety of fields, including metabolism, immunology, and drug development. Scientists are working to better understand the function of Adpgk in these contexts and explore its potential as a therapeutic target.

Can Adpgk be used as a potential drug target for metabolic disorders? 10/11/2019

There is growing interest in Adpgk as a potential drug target for metabolic disorders. Some studies have suggested that modulation of Adpgk activity could help regulate glucose metabolism, improve insulin sensitivity, and reduce the risk of metabolic diseases such as obesity and diabetes. However, further research is needed to fully explore the therapeutic potential of Adpgk in this context.

Where is Adpgk expressed in the body? 04/16/2019

Adpgk is expressed in a variety of tissues throughout the body, with highest expression levels observed in the liver, muscle, and brain. It is also present in adipose tissue, kidney, heart, and pancreas, although to a lesser extent. The widespread expression of Adpgk suggests that it plays a role in multiple physiological processes and that dysregulation of its expression or activity could contribute to the development of various diseases.

How is Adpgk regulated? 01/25/2019

Adpgk expression and activity are regulated by a variety of factors, including hormonal signals, nutrient availability, and oxidative stress. Insulin and glucagon are two key hormones that regulate Adpgk expression in response to changes in blood glucose levels. Nutrient availability also plays a role in Adpgk regulation, with high glucose levels leading to increased Adpgk expression. Furthermore, Adpgk activity can be modulated by post-translational modifications such as phosphorylation, acetylation, and ubiquitination. Finally, oxidative stress has been shown to downregulate Adpgk expression, possibly through the activation of stress-responsive pathways such as NF-κB.

Is Adpgk a potential target for drug development? 03/01/2018

Yes, Adpgk has been identified as a potential target for drug development in the treatment of metabolic disorders such as diabetes. Researchers are exploring the use of Adpgk activators or inhibitors to modulate glucose metabolism and improve glycemic control in patients with these conditions. However, further research is needed to determine the safety and efficacy of these approaches.

How is Adpgk studied in the laboratory? 02/09/2018

Adpgk can be studied in the laboratory using a variety of techniques, including molecular biology, biochemistry, and cell biology approaches. These may involve cloning and expressing Adpgk in bacterial or eukaryotic cells, purifying the protein to determine its structure and enzymatic activity, or using gene knockdown or overexpression techniques to investigate its function in cells or animal models. In addition, high-throughput screening methods such as chemical compound libraries can be used to identify small molecules that modulate Adpgk activity or expression.

What are the functions of Adpgk? 06/28/2017

Adpgk is involved in several cellular processes, including glucose metabolism, glycolysis, and glycogen synthesis. Its primary function is to catalyze the conversion of ADP-glucose (ADPG) to glucose-1-phosphate (G1P), which is an important step in glycogen synthesis. It also plays a role in regulating glucose metabolism and insulin signaling by modulating the levels of ADPG and G1P. In addition, Adpgk has been implicated in other processes such as DNA repair, lipid metabolism, and autophagy.

Are there any medical conditions associated with Adpgk mutations? 04/19/2017

Yes, mutations in the Adpgk gene have been linked to several medical conditions, including glycogen storage disease type 0 and type 13. These conditions are characterized by a deficiency in Adpgk activity, which impairs the body's ability to synthesize glycogen and regulate blood glucose levels. Symptoms of these conditions may include hypoglycemia, muscle weakness, and fatigue.

Can Adpgk be used as a biomarker for disease? 06/30/2016

There is some evidence to suggest that Adpgk levels may be altered in certain disease states, such as diabetes and liver disease. However, more research is needed to determine whether Adpgk could be used as a reliable biomarker for these conditions.

Are there any drugs that target Adpgk? 03/16/2016

Currently, there are no drugs that specifically target Adpgk. However, some compounds have been identified that can modulate Adpgk activity or expression indirectly. For example, some studies have found that certain bioactive compounds, such as berberine and quercetin, can induce Adpgk expression in cells and potentially have therapeutic benefits.

Customer Reviews (4)

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Reviews
06/10/2022

    I highly recommend this protein and the manufacturer's services to anyone in need of high-quality research materials and excellent technical support.

    12/06/2021

      I am very pleased with the excellent technical support provided by the manufacturer, which has been instrumental in solving some of my experimental problems.

      09/12/2017

        The ADPGK protein that I have obtained for my experiments is of exceptional quality and meets my experimental needs perfectly.

        11/21/2016

          Their prompt response and knowledge have been invaluable and have contributed significantly to the success of my research.

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