Recombinant Human ADH1B 293 Cell Lysate
Cat.No. : | ADH1B-9014HCL |
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- Gene Information
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Description : | Antigen standard for alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ADH1B alcohol dehydrogenase 1B (class I), beta polypeptide [ Homo sapiens ] |
Official Symbol : | ADH1B |
Synonyms : | ADH1B; alcohol dehydrogenase 1B (class I), beta polypeptide; ADH2; alcohol dehydrogenase 1B; ADH, beta subunit; aldehyde reductase; alcohol dehydrogenase subunit beta; alcohol dehydrogenase 2 (class I), beta polypeptide; |
Gene ID : | 125 |
mRNA Refseq : | NM_000668 |
Protein Refseq : | NP_000659 |
UniProt ID : | P00325 |
Chromosome Location : | 4q23 |
Pathway : | Biological oxidations, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Ethanol oxidation, organism-specific biosystem; Fatty Acid Omega Oxidation, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; |
Function : | alcohol dehydrogenase activity, zinc-dependent; metal ion binding; nucleotide binding; oxidoreductase activity; zinc ion binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionADH1B deficiency or knockout in experimental models leads to altered alcohol metabolism and related phenotypes, such as decreased alcohol clearance and increased sensitivity to alcohol-induced effects.
ADH1B plays a significant role in alcohol metabolism, showing specific catalytic efficiency and substrate specificity for the conversion of alcohol to acetaldehyde.
Pharmacological agents or environmental factors can modulate ADH1B expression or activity, potentially influencing alcohol metabolism and providing avenues for therapeutic interventions or personalized medicine.
Genetic variations or polymorphisms in the ADH1B gene have been associated with differences in alcohol metabolism and susceptibility to alcohol-related disorders, highlighting the impact of genotype on phenotype.
ADH1B has the capacity to metabolize other substrates beyond alcohol, suggesting its involvement in broader metabolic pathways and potential metabolic interactions.
ADH1B expression or activity may vary among different species or populations, influenced by genetic and environmental factors, contributing to inter-individual or inter-species differences in alcohol metabolism and tolerance.
ADH1B exhibits a tissue-specific expression pattern, with higher expression levels in certain organs such as the liver, and its distribution may differ from other ADH isoforms, reflecting functional specialization.
The structural features of ADH1B, such as its active site and binding domains, are critical for its enzymatic activity and substrate recognition, enabling efficient alcohol oxidation.
There may be interacting proteins or partners of ADH1B that influence its function or localization, potentially modulating its enzymatic activity or cellular processes.
The expression of ADH1B is regulated by specific mechanisms, including transcriptional and post-transcriptional regulation, which can respond to alcohol exposure or metabolic factors such as hormonal signaling.
Customer Reviews (2)
Write a reviewShows outstanding biocompatibility and low cytotoxicity in cell culture experiments.
Displays excellent resistance to denaturation by chaotropic agents in stability assays.
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