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Recombinant Human ACSBG1 293 Cell Lysate

Cat.No. : ACSBG1-9079HCL
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  • Gene Information
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Description : Antigen standard for acyl-CoA synthetase bubblegum family member 1 (ACSBG1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ACSBG1 acyl-CoA synthetase bubblegum family member 1 [ Homo sapiens ]
Official Symbol : ACSBG1
Synonyms : ACSBG1; acyl-CoA synthetase bubblegum family member 1; long-chain-fatty-acid--CoA ligase ACSBG1; BG1; BGM; bubblegum; FLJ30320; hBG1; hsBG; KIAA0631; lipidosin; MGC14352; very long chain acyl CoA synthetase; very long-chain acyl-CoA synthetase; BG; LPD; GR-LACS;
Gene ID : 23205
mRNA Refseq : NM_001199377
Protein Refseq : NP_001186306
MIM : 614362
UniProt ID : Q96GR2
Chromosome Location : 15q23-q24
Pathway : Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; PPAR signaling pathway, organism-specific biosystem; PPAR signaling pathway, conserved biosystem;
Function : ATP binding; ligase activity; long-chain fatty acid-CoA ligase activity; nucleotide binding; very long-chain fatty acid-CoA ligase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What is the relationship between ACSBG1 and insulin resistance? 10/05/3020

ACSBG1 knockout in animal models leads to improved insulin sensitivity, and ACSBG1 expression is upregulated in insulin-resistant states.

What is the primary tissue distribution of ACSBG1 expression? 04/09/2022

ACSBG1 is primarily expressed in the liver and adipose tissue, but is also found in other tissues such as the brain.

What is the role of ACSBG1 in insulin signaling? 07/13/2021

ACSBG1 is involved in insulin signaling by regulating the levels of diacylglycerols, which can activate PKCε and inhibit insulin signaling.

What is the mechanism of ACSBG1 regulation by PGC-1α? 06/17/2021

PGC-1α regulates ACSBG1 expression by interacting with PPARα to increase transcription, as well as by promoting the acetylation of histones in the ACSBG1 promoter region.

What is the role of ACSBG1 in cancer? 11/07/2020

ACSBG1 has been found to be upregulated in several types of cancer, including breast, prostate, and liver cancer, and may play a role in tumor growth and progression.

What is the role of ACSBG1 in lipid droplet formation? 08/18/2020

ACSBG1 is involved in the formation of lipid droplets by promoting the esterification of fatty acids into triacylglycerols.

How is the expression of ACSBG1 regulated? 07/11/2020

ACSBG1 expression is regulated by a number of transcription factors, including SREBP1c, PPARα, and PGC-1α, which are all involved in regulating lipid metabolism.

What is the role of ACSBG1 in non-alcoholic fatty liver disease (NAFLD)? 03/11/2019

ACSBG1 expression is upregulated in the liver of individuals with NAFLD, and ACSBG1 knockout in animal models leads to reduced hepatic steatosis.

What is the mechanism of ACSBG1 regulation by PPARα? 03/10/2019

PPARα regulates ACSBG1 expression by binding to a specific site in the ACSBG1 promoter and increasing transcription.

What is the substrate specificity of ACSBG1? 05/18/2018

ACSBG1 has a broad substrate specificity and can activate a variety of long-chain fatty acids.

Customer Reviews (5)

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Reviews
07/19/2020

    The samples provided by the manufacturer allowed me to test out different proteins before committing to a larger purchase, which was extremely helpful.

    04/23/2020

      The manufacturer's consistency in delivering high quality proteins has made them my go-to supplier.

      08/16/2019

        I was impressed by the follow-up from the manufacturer's after-sales team, who ensured that I was satisfied with my purchase.

        07/09/2019

          A satisfying purchase

          07/13/2018

            All good. Will recommand to my other colleague

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