"ACSBG1" Related Products

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Recombinant Human ACSBG1 293 Cell Lysate

Cat.No.: ACSBG1-9079HCL
Description: Antigen standard for acyl-CoA synthetase bubblegum family member 1 (ACSBG1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ACSBG1 acyl-CoA synthetase bubblegum family member 1 [ Homo sapiens ]
Official Symbol: ACSBG1
Synonyms: ACSBG1; acyl-CoA synthetase bubblegum family member 1; long-chain-fatty-acid--CoA ligase ACSBG1; BG1; BGM; bubblegum; FLJ30320; hBG1; hsBG; KIAA0631; lipidosin; MGC14352; very long chain acyl CoA synthetase; very long-chain acyl-CoA synthetase; BG; LPD; GR-LACS;
Gene ID: 23205
mRNA Refseq: NM_001199377
Protein Refseq: NP_001186306
MIM: 614362
UniProt ID: Q96GR2
Chromosome Location: 15q23-q24
Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; PPAR signaling pathway, organism-specific biosystem; PPAR signaling pathway, conserved biosystem;
Function: ATP binding; ligase activity; long-chain fatty acid-CoA ligase activity; nucleotide binding; very long-chain fatty acid-CoA ligase activity;

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