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Recombinant Human ACP1 293 Cell Lysate

Cat.No. : ACP1-9083HCL
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  • Gene Information
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Description : Antigen standard for acid phosphatase 1, soluble (ACP1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ACP1 acid phosphatase 1, soluble [ Homo sapiens ]
Official Symbol : ACP1
Synonyms : ACP1; acid phosphatase 1, soluble; low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; LMW-PTPase; adipocyte acid phosphatase; red cell acid phosphatase 1; protein tyrosine phosphatase; acid phosphatase of erythrocyte; cytoplasmic phosphotyrosyl protein phosphatase; low molecular weight cytosolic acid phosphatase; HAAP; MGC3499; MGC111030;
Gene ID : 52
mRNA Refseq : NM_004300
Protein Refseq : NP_004291
MIM : 171500
UniProt ID : P24666
Chromosome Location : 2p25
Pathway : Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; EPHA2 forward signaling, organism-specific biosystem; PDGFR-beta signaling pathway, organism-specific biosystem; Riboflavin metabolism, organism-specific biosystem; Riboflavin metabolism, conserved biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem;
Function : acid phosphatase activity; hydrolase activity; non-membrane spanning protein tyrosine phosphatase activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What are the two subtypes of ACP1? 02/14/2023

F and S.

How to analyze ACP1 polymorphism? 02/14/2023

Through the polymerase chain reaction-resreiction fragment length polymorphism.

What disease would be influenced by ABHD14A? 02/13/2023

Esophageal cancer, gastric cancer and gastrointestinal cancer will lead to up-regulation of ACP1 expression, while colorectal cancer will reduce it.

Why is the blood glucose level of inactive ACP1 A low? 02/11/2023

Because ACP1 has low dephosphorylation effect on insulin receptor.

How ACP1 works on respiration and cell oxidation? 02/10/2023

To adjust the level of FMN and flavin adenine dinucleotide.

How to detect the protein level of ACP1? 02/08/2023

Immunohistochemical staining observation of two related protein sections.

How many codominant alleles are there in ACP1? 02/08/2023

Three.

What activities may ACP1 participate in the brain? 02/07/2023

The cholesterol biosynthesis, neutotransmitter synthesis and it links to behavioral and cellular response to stress via p190RhoGAP

What's the ACP1 blocking buffer? 02/05/2023

The PBS conraining 10% goat serum.

What protein is significantly associated with ACP1 in the brain? 02/05/2023

The TPH2, DDC and GAD1.

Customer Reviews (3)

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Reviews
02/17/2023

    The answers are very concise.

    02/16/2023

      There are all the questions I want to know.

      02/16/2023

        The answer was very patient.

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