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Recombinant Human ACAD8 293 Cell Lysate

Cat.No. : ACAD8-9116HCL
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  • Gene Information
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Description : Antigen standard for acyl-Coenzyme A dehydrogenase family, member 8 (ACAD8), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ACAD8 acyl-CoA dehydrogenase family, member 8 [ Homo sapiens ]
Official Symbol : ACAD8
Synonyms : ACAD8; acyl-CoA dehydrogenase family, member 8; acyl Coenzyme A dehydrogenase family, member 8; isobutyryl-CoA dehydrogenase, mitochondrial; activator-recruited cofactor 42 kDa component; acyl-Coenzyme A dehydrogenase family, member 8; ARC42; ACAD-8;
Gene ID : 27034
mRNA Refseq : NM_014384
Protein Refseq : NP_055199
MIM : 604773
UniProt ID : Q9UKU7
Chromosome Location : 11q25
Pathway : Branched-chain amino acid catabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Valine, leucine and isoleucine degradation, organism-specific biosystem; Valine, leucine and isoleucine degradation, conserved biosystem; valine degradation I, organism-specific biosystem;
Function : acyl-CoA dehydrogenase activity; acyl-CoA dehydrogenase activity; flavin adenine dinucleotide binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What is the primary structure of acyl-CoA dehydrogenase family member 8 protein? 06/10/2022

The primary structure of acyl-CoA dehydrogenase family member 8 protein consists of a chain of amino acids.

Are there any drugs or compounds that target acyl-CoA dehydrogenase family member 8 protein for therapeutic purposes? 04/14/2022

There are no known drugs or compounds that specifically target acyl-CoA dehydrogenase family member 8 protein for therapeutic purposes.

What are the biochemical pathways that acyl-CoA dehydrogenase family member 8 protein is involved in? 01/25/2022

Acyl-CoA dehydrogenase family member 8 protein is involved in multiple biochemical pathways.

What is the three-dimensional structure of acyl-CoA dehydrogenase family member 8 protein? 12/13/2021

The three-dimensional structure of acyl-CoA dehydrogenase family member 8 protein is currently unknown.

What is the molecular weight of acyl-CoA dehydrogenase family member 8 protein? 11/06/2021

The molecular weight of acyl-CoA dehydrogenase family member 8 protein is approximately 75 kDa.

What is the function of acyl-CoA dehydrogenase family member 8 protein? 07/09/2021

The function of acyl-CoA dehydrogenase family member 8 protein is involved in fatty acid metabolism.

Are there any known genetic variations or mutations in acyl-CoA dehydrogenase family member 8 protein associated with disease susceptibility? 03/22/2021

There are genetic variations or mutations in acyl-CoA dehydrogenase family member 8 protein associated with disease susceptibility.

What is the tissue distribution of acyl-CoA dehydrogenase family member 8 protein in humans? 04/16/2019

Acyl-CoA dehydrogenase family member 8 protein is expressed in various human tissues.

What are the known interacting proteins or molecules of acyl-CoA dehydrogenase family member 8 protein? 02/09/2018

There are known interacting proteins or molecules of acyl-CoA dehydrogenase family member 8 protein.

What is the role of acyl-CoA dehydrogenase family member 8 protein in human diseases? 12/03/2017

The role of acyl-CoA dehydrogenase family member 8 protein in human diseases is currently being studied.

Customer Reviews (2)

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Reviews
08/17/2021

    Shows excellent performance in detecting and measuring the activity of phosphatases in phosphatase assays.

    08/16/2017

      Highly effective in detecting and quantifying the expression levels of fusion proteins in protein expression analysis.

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