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Recombinant Human ABI1 293 Cell Lysate

Cat.No. : ABI1-9128HCL
  • Specification
  • Gene Information
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Description : Antigen standard for abl-interactor 1 (ABI1), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ABI1 abl-interactor 1 [ Homo sapiens ]
Official Symbol : ABI1
Synonyms : ABI1; abl-interactor 1; spectrin SH3 domain binding protein 1 , SSH3BP1; abl interactor 1; ABI 1; E3B1; Abelson interactor 1; nap1 binding protein; abl-binding protein 4; interactor protein AblBP4; Abl-interactor protein 1 long; eps8 SH3 domain-binding protein; spectrin SH3 domain-binding protein 1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1;
Gene ID : 10006
mRNA Refseq : NM_001012752
Protein Refseq : NP_001012770
MIM : 603050
UniProt ID : Q8IZP0
Chromosome Location : 10p12.1
Pathway : E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; RAC1 signaling pathway, organism-specific biosystem; Regulation of RAC1 activity, organism-specific biosystem; Stabilization and expansion of the E-cadherin adherens junction, organism-specific biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem;
Function : cytoskeletal protein binding; protein binding; protein tyrosine kinase activator activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Is there a TEV cleavage site between the GST tag and the ABI1 protein sequence? 04/14/2023

Some of our products contain cleavage sites between the tag and the target protein. Please consult our experts for specific product information.

Can you express the ABI1 fusion protein with MBP tag? 04/14/2023

Sure, Creative BioMart supports custom protein production services.

Which expression systems does recombinant ABI1 protein use? 04/14/2023

We provide various expression systems, including E. coli, wheat germ, in vitro cell free system, and mammalian cell expression systems.

What signaling pathway is ABI1 involved in? 04/14/2023

ABI1 may play a role in regulating EGF-induced activation of the Erk pathway and, together with EPS8, is involved in signal transduction from Ras to Rac.

Can your ABI1 proteins be used for WB? 04/14/2023

Yes, our recombinant ABI1 protein can be used as a variety of applications based on the antigen-antibody reaction.

Customer Reviews (2)

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Reviews
06/14/2020

    Recombinant chicken ABI1 has high purity and good immunogenicity, and the experimental results are very satisfactory.

    05/13/2018

      The technical support experts at Creative BioMart customized the ABI1 protein with a specific expression system and fusion tag for me.

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