Recombinant Human MMP3

• Cat.No.: MMP3-39H
• Product Overview: Recombinant Human Matrix Metalloproteinase-3 Human Recombinant produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 230 amino acids and having a molecular mass of 22 kDa. The MMP-3 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: MMP3-39H
• Description: MMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk . MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1 mg/ml) solution with no additives.
• Biological Activity: The specific activity was found to be ≥1,250 mU/mg protein (H. Nagase, et al; J. Biol. Chem. 269, 20952, 1994). One unit is defined as the amount of enzyme that hydrolyzes 1µmol of MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH2 per min. at 37°C, pH 7.0.
• Solubility: It is recommended to reconstitute the lyophilized Stromelysin-1 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized Matrix Metalloproteinase-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MMP-3 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: MMP3 matrix metallopeptidase 3 (stromelysin 1, progelatinase) [ Homo sapiens ]
• Synonyms: matrix metallopeptidase 3 (stromelysin 1, progelatinase); SL-1; STMY; STR1; CHDS6; MMP-3; STMY1; MGC126102; transin-1; MMP3; MGC126103; matrix metalloproteinase 3; CHDS6; Transin-1; proteoglycanase; MGC126104; Matrix metalloproteinase-3; EC 3.4.24.17
• Gene ID: 4314
• mRNA Refseq: NM_002422
• Protein Refseq: NP_002413
• MIM: 185250
• UniProt ID: P08254
• Chromosome Location: 11q22.3
• Function: calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding