Recombinant Human FZD5 protein, Fc-tagged

• Cat.No.: FZD5-167H
• Product Overview: Recombinant Human FZD5 (Ala27-Pro167) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Frizzled-5 (FZD5) is also known as FzE5, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD5 contains one FZ (frizzled) domain. FZD5 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD5 interacts specifically with Wnt5A to induce the beta-catenin pathway. FZD5 interacts with GOPC.
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Predicted N Terminal: Ala27
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 42.6 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ala27-Pro167
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FZD5
• Official Symbol: FZD5
• Synonyms: FZD5; frizzled family receptor 5; C2orf31, chromosome 2 open reading frame 31, frizzled (Drosophila) homolog 5, frizzled 5, seven transmembrane spanning receptor, frizzled homolog 5 (Drosophila); frizzled-5; DKFZP434E2135; HFZ5; fz-5; fzE5; Wnt receptor; seven-transmembrane receptor frizzled-5; frizzled 5, seven transmembrane spanning receptor; C2orf31; MGC129692; DKFZp434E2135
• Gene ID: 7855
• mRNA Refseq: NM_003468
• Protein Refseq: NP_003459
• MIM: 601723
• UniProt ID: Q13467