Recombinant Human AGO2, His-tagged

• Cat.No.: AGO2-2180H
• Product Overview: Recombinant Human AGO2 (NP_036286.2) full length (Met 1-Ala 859), fused with a polyhistidine tag at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect Cell
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human AGO2 consists of 877 amino acids and predicts a molecular mass of 99 kDa as estimated by SDS-PAGE under reducing conditions.
• Protein Length: Met 1-Ala 859
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Probing the nanoparticle–AGO2 interaction for enhanced gene knockdown (2018)