Recombinant Human KMT2D, GST-tagged

• Cat.No.: KMT2D-55H
• Product Overview: Recombinant Human MLL2 protein (accession number NP_003473.3) was expressed in E. coli, with an N-terminal GST tag.

Specification

• Description: Myeloid/lymphoid or mixed-lineage leukemia 2 (MLL2) is a Trithorax-group protein that function collectively to promote gene expression. MLL2 is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL2 is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the β-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 20 mM Tris-HCl pH 7.4, 150 mM NaCl and 20% glycerol.
• Molecular Mass: 47.6 kDa
• Applications: Enzyme kinetics, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.6 mg/ml