Active Recombinant Human ADAM10, His-tagged

• Cat.No.: ADAM10-18H
• Product Overview: Recombinant Human ADAM10(Thr214-Glu672), fused with a C-terminal 10-His tag, was expressed in Sf 21(baculovirus).

Specification

• Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin.
• Source: Sf 21 (baculovirus)
• Species: Human
• Tag: His
• Predicted N Terminal: Thr214
• Form: Recombinant Human ADAM10 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag. Supplied as a 0.2 µm filtered solution in MES, NaCl, ZnCl2,Glycerol and Brij-35.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2.
• Molecular Mass: Predicted Molecular Mass:52 kDaSDS-PAGE:60 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 °C as supplied.3 months, -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: ADAM10 ADAM metallopeptidase domain 10 [ Homo sapiens ]
• Official Symbol: ADAM10
• Synonyms: ADAM10; ADAM metallopeptidase domain 10; a disintegrin and metalloproteinase domain 10; disintegrin and metalloproteinase domain-containing protein 10; CD156c; HsT18717; kuz; MADM
• Gene ID: 102
• mRNA Refseq: NM_001110
• Protein Refseq: NP_001101
• MIM: 602192
• UniProt ID: O14672
• Chromosome Location: 15q22
• Pathway: Activated NOTCH1 Transmits Signal to the Nucleus; Collagen degradation; Constitutive Signaling by NOTCH1 PEST Domain Mutants
• Function: SH3 domain binding; endopeptidase activity; metalloendopeptidase activity