||Fibroblast Growth Factor-acidic Bovine (FGF-1) purified from Bovine Brain contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20K peptide by restricted proteolysis. The FGF acidic is purified by proprietary chromatographic techniques.
||Each 5μg aFGF were lyophilized from 0.5ml solution containing 1mM sodium phosphate, pH 7 after filtration over a low binding membrane.
||The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is< 2.0 ng/ml, corresponding to a specific activity of>500,000 units/mg.
||Greater than 90%.
||This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
||Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution aFGF should be stored at 4°C between 2-3 weeks and for future use below -18°C.Please prevent freeze-thaw cycles.
||It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.