Active Native Porcine Porcine Calpain 1

• Cat.No.: CAPN1-65P

Specification

• Description: The activity of Calpain activity is attributed to two main isoforms: n-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (n-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.
• Source: Porcine Erythrocytes
• Species: Porcine
• Form: Liquid
• Bio-activity: ≥ 1000 units/mg protein prior to freezing.
• Molecular Mass: 110 kDa
• Purity: Purity by SDS-PAGE: ≥85%
• Usage: For Research Use Only! Not For Use in Humans.
• Notes: Centrifuge the vial prior to opening
• Storage: -70°C
• Storage Buffer: In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
• Shipping: Gel pack

Gene Information

• Gene Name: calpain 1, (mu/I) large subunit [ Sus scrofa ]
• Official Symbol: CAPN1
• Synonyms: calpain-1 catalytic subunit; CANP 1; muCANPcalpain mu-type; micromolar-calpain; calpain-1 large subunit; calcium-activated neutral proteinase 1; micromolar calcium-activated neutral protease 1
• Gene ID: 397027
• mRNA Refseq: NM_213972
• Protein Refseq: NP_999137
• Chromosome Location: chromosome: 2
• Pathway: Alzheimer"s disease, organism-specific biosystem; Alzheimer"s disease, conserved biosystem; Apoptosis, organism-specific biosystem
• Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity